Asian Journal of Food Research and Nutrition

Green Synthesis of Zinc Oxide Nanoparticles Using Colocasia esculenta Tuber Peel Extract and Antimicrobial Studies of White Yam Pathogens

Ugosor Paul Terngu *

Department of Chemistry, College of Education, Katsina-Ala, Benue State, Nigeria.

Anhwange Benjamin Asen

Department of Chemistry, Benue State University, Makurdi, Nigeria.

Okibe Friday Godwin

Department of Chemistry, Federal University of Health Sciences, Oturkpo, Benue State, Nigeria.

Dooshima Shiriki

Department of Biological Sciences, Benue State University, Makurdi, Nigeria.

*Author to whom correspondence should be addressed.

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Abstract

Aims: To biosynthesize and characterize Zinc Oxide NPs using Colocasia esculenta (Cocoyam) tuber peel extract as well as explores its antimicrobial potential against white yam pathogens.

Place and Duration of Study: Department of Chemistry, Benue State University, Makurdi, November, 2022.

Methodology: The method described by Nakade [1] was used for phytochemIcal analysis (Tanins, Saponins, Flavonoids, Alkaloids, Steriods, Quinones, Starch, Terpenoids and Glycosides).The biosynthesized ZnO NPs were characterized by  UV-Visible, XRD, SEM, EDX and FTIR.

Antimicrobial sensitivity test was by the method described by Shiriki et al [2] with slight modification.

Results: Optimization studies revealed that the maximum rate of synthesis could be achieved with 0.50 M ZnO solution at 90 ^oC in 5 hours. The study revealed ZnO NPs that are crystalline with hexagonal shapes. The average crystallite size was 10 nm with a range of 7.81 nm- 9.23 nm. FTIR spectra of the tuber peel extract and the synthesized ZnO NPs revealed reducing, capping and stabilizing agents such as amines, peptides, amides and phenolic groups. The biosynthesized ZnO NPs exhibited antimicrobial action in a dose-dependent manner against five white yam pathogenic fungi: Aspergillus niger, Botryodioplodia theobromae, Zygosaccharomyces bailli, Zygosaccharomyces rouxil, and Myrothecium verrucaria as well as three bacteria: Klebsiella oxytoca, Serratia marcenscens, and Pseudomonas aeruginosa.

Conclusion: The biosynthesized ZnO NPs exhibited slightly to moderate and effective inhibition ranging from 15.54%- 98.52% on the test organisms when compared with standard antifungal (Fluconazole) and antibacterial (Ciprofloxacin) agents.

Keywords: Phytochemicals, nanotechnology, biosynthesis, pathogens, antimicrobial activity

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References

Nakade DB, Mahseh SK, Kiran NP, Vinayak SM. Phytochemical screening and Antibacterial Activity of Western Region wild leaf Colocasea esculenta. International Research Journal of Biological Science. 2013;2(10):1-6.

Shiriki D, Obochi GO, Eke MO, Shambe T. Postharvest Loss Control: Synergistic Plants Extract Inhibition of Ten Microbial Yam Rot Organisms. 2017;8(7):25-732 Available:https://doi.org/10.4236/fns.2017.87051

Jayanta KB. Synthesis and characteriztion of zno nanoparticles. a master of science dissertation of the department of physics. National Institute of Technology, Rourkela, Orissa, India; 2020.

Josef J, Katarina K. Application of nanotechnology in agriculture and food industry, its prospects and risks .Ecol chem Eng s. 2015;22(3):321-361.

Ahmed SA, Taia A, Ahmad OB,Samy S, Abir MH. A.Green Synthesis and Characterization of ZnO Nanoparticles Using Pelargonium odoratissimum(L.) Aqueous Leaf Extract and Their Antioxidant, Antibacterial and Anti-inflammatory Activities. Antioxidants (Basel).2022;(8): 1444.

DOI:10.3390/antiox11081444

Mittal AK, Chisti Y, Banerjee UC.Synthesis of metallic nanoparticles using plant extracts Biotechnol Adv; 2013 DOI: 10.1016/j.biotechadv.2013.01.003

Kharissova OV, Dias HVR, Kharisov BI, Perez BO, Perez VMJ. The greener synthesis of nanoparticles Trends in Biotechnology. 2013;31(4):240-48.

Yasser S, Nassim S. Current advances in applications of chitosan based nanaometal oxides as food preservative materials. Nanomed J. 2019;4:122-129.

Zare E, Pourseyedi S, Khatami M, Darezereshki E. Simple biosynthesis of zinc oxide nanoparticles using nature’s source, and it’s in vitro bio-activity. Journal of Molecular Structure. 2017;1146:96-103.

Eleazu C0. Characterization of the natural products in cocoyam (Colocasia esculenta) using GC-MS. Pharmaceutical Biology. 2016;54(12):2880-2885.

Wang JK. Taro-a review of Colocasia esculenta and its potentials. Journal of Biotechnology and Pharmaceutical Research. 2012;3:42-46.

Pritha C, Papiya D, Sudeshna C, Bohniskilda C, Jayantihi A. Cytotoxicity and antimicrobial activity of Colocasea esculenta. Journal of Chemical and Pharmaceutical Research. 2015;7(12):627-635.

Lakshmi JV, Sharath R, Chandraprabha MN, Neelufar E, Hazra, Abhishikta, Patra, Malyasree Synthesis, characterization and evaluation of antimicrobial activity of zincoxide nanoparticles. J. Biochem. Technology. 2012;3(5):S151–S154.

Priyatharesini PI, Ganesamoorthy R, Sudha R. Synthesis of zinc oxide nanoparticle using Cocos nucifera male flower extract and analysis of their antimicrobial Activity. J of Pharm andTech. 2020;13:2151- 2154.

Sangeetha G, Rajeshwari S, Venckatesh R. Green synthesis of zinc oxide nanoparticles by aloe barbadensis miller leaf extract: Structure and optical properties. Mater. Res. Bull. 2011;46:2560–2566.

Vijayakumar S, Vinoj G, Malaikozhunddan B, Shanthi S, Vaseeharan B. Plectranthus amboinicus leaf extract mediated synthesis of zinc oxide nanoparticles and its control of methacillin resistant Staphylococus aureus biofilm and blood sucking mosquito larva. Spectrochim. Acta Part Amol. Biomol. Spectrose. 2015; 137:886-891 Available:http://dx.doi.org/10.1016/j.saa.2014.08.064.

Elumalai K, Velmurugan S. Green synthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from the leaf extract of Azadirachta indica (L) Applied Surface Science. 2015;345:329-36.

Divya MJ, Sowmia C, Joona K, Dhanya KP. Synthesis of zinc oxide nanoparticle from Hibiscus rosa-sinensis leaf extract and investigation of its antimicrobial activity. Res; 2013.

Rahayu V, Wonoputri V, Nad Samadhi TW. Plant extract-assisted biosynthesis of zinc oxide nanoparticles and their antibacterial applications. Material Science and Engineering. 2020;823;012-036.

Jha AK, Kumar V, Prasad K. Biosynthesis of metal and oxide nanoparticles using orange juice. J. Bionanoscience. 2011;5(2):162–166. Available:http://dx.doi.org/10.1166/jbns.2011.1053

Rad SS, Sani AM, Mohseni S. Biosynthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from leaf extract of Mentha pulegium (L.),Microbial Pathogenesis. 2019;131(2019):239–245.

Padil VVT, Cernik M. Green synthesis of copper oxide nanoparticles using gum karaya as biotemplate and their antibacterial application. International Journal of Nanomedicine. 2013;8:889-98.

Rajiv P, Rajeshwari S, Venckatesh R. Rambutan peels promoted biomimetic synthesis of bioinspired zinc oxide nanochains for biomedical applications. Spectrochim. Acta Part A Mol.Biomol.Spectros. 2013;112:384–387. Available:http://dx.doi.org/10.1016/j.saa.2014.08.022

Moloto N, Revaprasadu N, Musetha PL, Moloto MJ. The effect of precursor concentration, temperature and capping group on the morphology of CdS nanoparticles. Journal of Nanoscience and Nanotechnology. 2009;9:4760-66.

Sibiya PN, Moloto MJ. Effect of precursor concentration and pH on the shape and size of starch capped silver selenide (Ag2Se) nanoparticles. Chalcogenide Letters. 2014;11(11):577-88.

Amin G, Asif MH, Zainelabidin A, Zaman S, Nur O, Willander M. Influence of Ph, precursor concentration, growth time, and temperature on the morphology of Zno nanostructures grown by the hydrothermal method. Journal of Nanomaterials. 2011;269692.

Shiriki D, Ubwa ST, Yusufu MI, Shambe T. Extraction methods and inhibition studies of ten plant extracts on nine yam rot pathogenic microorganisms.Food and Nutrition Sciences; 2019.

Available:https://doi.org10.4236/fns.2019

Farjana R, Md Abdul MP, Md Abu BS, Muhammad SB, Md. Aminul H, Beauty A, Rimi R, Md. Anamul H, Royhan AKM. Green synthesis of ZnO nanoparticles using Cocos nucifera leaf extract: Characterization, antimicrobial, antioxidant, and photocatalytic activity; 2022 Available:https//doi.org/10.11012020.10.27.514023.

Ramesh P, Rajendran A, Meenakshisundaram M. Green synthesis of zinc oxide nanoparticles using flower extract Cassia auriculata. J NS NT. 2014;1(1):41–45.

ISSN 2279 –0381

Parveen K, Banse V, Ledwani L. Green synthesis of nanoparticles: Their advantages and disadvantages 2nd International Conference on Emerging Technologies: Micro to Nano; 2015 (ETMN-2015) DOI: 10.1063/1.4945168

Salam AH, Sivaraj R, Venckatesh R. Green synthesis and characterization of zinc oxide nanoparticles from Ocimum basilicum, L. var. purpurascens, Benth.-Lamiaceae leaf extract. Mater.Lett. 2014;131:16–18.

Available:http://dx.doi.org/10.1016/j.matlet.2014.05.033

Sathishkumar G, Rajkuberan C, Manikandan K, Prabukumar S, DanielJohn J, Sivaramakrishnan S. Facile biosynthesis of antimicrobial zinc oxide (ZnO) Nano flakes using leaf extract of Couroupitaguianensis.Aubl, Mater. Lett. 2017;188:383–386.