In vivo Evaluation of the Anticancer Efficacy of Polysaccharide Extracted from the Fruiting Bodies of Pleurotus Ostreatus

Md Moyen Uddin PK *

Institute of Biological Sciences, Rajshahi University, Bangladesh.

Mohammad Sayful Islam

Department of Pharmacy, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh.

Mohammad Shahangir Biswas

Department of Biochemistry and Biotechnology, School of Biomedical Science, Khwaja Yunus Ali Unversity, Enayetpur, Sirajgonj-6751, Bangladesh.

Rumana Pervin

Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.

Matiar Rahman

Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.

*Author to whom correspondence should be addressed.


This study investigated the effect of Pleurotus ostreatus polysaccharide (POP) on Ehrlich Ascites Carcinoma (EAC) cells both In vitro and In vivo. In vitro analysis revealed a dose-dependent decrease in EAC cell viability with increasing concentrations of POP, with pronounced cytotoxic effects observed at concentrations exceeding 150 μg/mL. In vivo studies demonstrated that POP treatment significantly extended lifespan and mitigated tumor-induced weight loss compared to untreated EAC groups. Furthermore, POP treatment exhibited a substantial inhibitory effect on tumor growth, as evidenced by reductions in tumor weight and volume. Hematological and biochemical analyses indicated that POP treatment reversed tumor-induced alterations in hematological parameters and ameliorated biochemical markers associated with cellular damage. EAC and EAC+Bleomycin decreased hemoglobin, red blood cell count, and platelet count, while EAC increased white blood cell count. Conversely, POP(4xIC50) caused slight increases in these parameters, suggesting milder cellular damage. On the other hand, the elevated levels of CK-MB, LDH, AST, ALT, ALP, Creatinine, and BUN indicated cellular damage in groups treated with EAC, EAC+Bleomycin, and POP(4xIC50) compared to normal. These markers suggested myocardial, tissue, liver, bone, and kidney damage, highlighting the adverse effects of these treatments on cellular health and organ function. Histological examination revealed a protective effect of POP on cardiac, hepatic, and renal tissues, suggesting its potential in preserving organ function. These findings underscored the therapeutic potential of POP in targeting cancer cells and mitigating tumor-induced physiological and pathological changes.

Keywords: Pleurotus ostreatus polysaccharide, Ehrlich Ascites Carcinoma, anti-tumor effects, cytotoxicity, tumor growth inhibition

How to Cite

Uddin PK , M. M., Islam, M. S., Biswas, M. S., Pervin, R., & Rahman, M. (2024). In vivo Evaluation of the Anticancer Efficacy of Polysaccharide Extracted from the Fruiting Bodies of Pleurotus Ostreatus. Asian Journal of Food Research and Nutrition, 3(3), 457–470. Retrieved from


Download data is not yet available.


Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. International Journal of cancer. 2015;136 (5):E359-86.

Turan I, Demir S, Misir S, Kilinc K, Mentese A, Aliyazicioglu Y, Deger O. Cytotoxic effect of Turkish propolis on liver, colon, breast, cervix and prostate cancer cell lines. Tropical Journal of Pharmaceutical Research. 2015;14(5): 777-82.

Pang CL, Zhang X, Wang Z, Ou J, Lu Y, Chen P, Zhao C, Wang X, Zhang H, Roussakow SV. Local modulated electro-hyperthermia in combination with traditional Chinese medicine vs. intraperitoneal chemoinfusion for the treatment of peritoneal carcinomatosis with malignant ascites: A phase II randomized trial. Molecular and clinical oncology. 2017; 6(5):723-32.

Liang Z, Guo YT, Yi YJ, Wang RC, Hu QL, Xiong XY. Ganoderma lucidum polysaccharides target a Fas/caspase dependent pathway to induce apoptosis in human colon cancer cells. Asian Pacific Journal of Cancer Prevention. 2014;15 (9):3981-6.

Gan QX, Wang J, Hu J, Lou GH, Xiong HJ, Peng CY, Huang QW. Modulation of apoptosis by plant polysaccharides for exerting anti-cancer effects: A review. Frontiers in Pharmacology. 2020; 11:792.

Friedman M. Mushroom polysaccharides: chemistry and antiobesity, antidiabetes, anticancer, and antibiotic properties in cells, rodents, and humans. Foods. 2016;5(4):80.

Kalmıs E, Azbar N, Yıldız H, Kalyoncu F. Feasibility of using olive mill effluent (OME) as a wetting agent during the cultivation of oyster mushroom, Pleurotus ostreatus, on wheat straw. Bioresource Technology. 2008;99(1):164-9.

Tong H, Xia F, Feng K, Sun G, Gao X, Sun L, Jiang R, Tian D, Sun X. Structural characterization and in vitro antitumor activity of a novel polysaccharide isolated from the fruiting bodies of Pleurotus ostreatus. Bioresource Technology. 2009;100(4): 1682-6.

Jayakumar T, Thomas PA, Geraldine P. Protective effect of an extract of the oyster mushroom, Pleurotus ostreatus, on antioxidants of major organs of aged rats. Experimental gerontology. 2007;42(3):183-91.

Adebayo EA, Oloke JK. Oyster mushroom (Pleurotus species); a natural functional food.

Pinheiro F, Faria RR, De Camargo JL, Spinardi-Barbisan AL, Da Eira AF, Barbisan LF. Chemoprevention of preneoplastic liver foci development by dietary mushroom Agaricus blazei Murrill in the rat. Food and Chemical Toxicology. 2003;41(11):1543-50.

Lavi I, Friesem D, Geresh S, Hadar Y, Schwartz B. An aqueous polysaccharide extract from the edible mushroom Pleurotus ostreatus induces anti-proliferative and pro-apoptotic effects on HT-29 colon cancer cells. Cancer letters. 2006;244(1):61-70.

A. Barnes D, Barlow R, Singh Nigam P, Owusu-Apenten R. Antioxidant, Anticancer and Antibacterial Activity of Withania somnifera Aqueous Root Extract. J. Adv. Biol. Biotechnol. 2015;5(1): 1-6.


[Accessed on:2024 May 28]

Vinitha V, Meignanalakshmi S, Tirumurugaan KG, Sarathchandra G, Sundaram SM. Poly-unsaturated Fatty Acid, Biodiesel Property and Anticancer Activity Analysis of Monoraphidium griffithii. Curr. J. Appl. Sci. Technol. 2022;41(41): 44-55.


Accessed on:2024 May 28];

Wang X, Yuan S, Wang J, Lin P, Liu G, Lu Y, Zhang J, Wang W, Wei Y. Anticancer activity of litchi fruit pericarp extract against human breast cancer in vitro and in vivo. Toxicology and applied pharmacology. 2006 Sep 1;215(2):168-78.

Uddin Pk MM, Islam MS, Pervin R, Dutta S, Talukder RI, Rahman M. Optimization of extraction of antioxidant polysaccharide from Pleurotus ostreatus (Jacq.) P. Kumm and its cytotoxic activity against murine lymphoid cancer cell line. PLoS One. 2019;14 (1):e0209371.

Mitry MA, Edwards JG. Doxorubicin induced heart failure: Phenotype and molecular mechanisms. IJC heart & vasculature. 2016;10:17-24.

dos Santos DS, dos Santos Goldenberg RC. Doxorubicin-induced cardiotoxicity: from mechanisms to development of efficient therapy. Cardiotoxicity. 2018:3-24.

Shivakumar P, Rani MU, Reddy AG, Anjaneyulu Y. A study on the toxic effects of doxorubicin on the histology of certain organs. Toxicology international. 2012;19 (3):241.