Application Natural Preservative Use Propolis: Effect on Reduction Sugar, Antioxidant and Physical of Milk Jelly Candy

Faradisa Zul Alfi Husna

Magister Student of Animal Science Faculty, Universitas Brawijaya, Malang 65145, Indonesia.

Dedes Amertaningtyas *

Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia.

Khothibul Umam Al Awwaly

Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia.

Herly Evanuarini

Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia.

Firman Jaya

Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia.

Aris Sri Widati

Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia.

Agus Susilo

Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia.

Premy Puspitawati Rahayu

Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia.

Aulia Puspita Anugra Yekti

Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia.

*Author to whom correspondence should be addressed.


Abstract

Aims: To determine the effect of adding propolis as a natural preservative on reduction sugar, antioxidant, water activity, texture and color (L a* b*) of milk jelly candy.

Study Design: This study used a Completely Randomized Design (CRD) with 5 treatments and 5 replications.

Place and Duration of Study: The research was conducted in the livestock technology laboratory of the Faculty of Animal Science, Brawijaya University, from August to October 2025.

Methodology: This research used the ANOVA test and DMRT follow-up test for analysis. The treatments in this study were the levels of propolis addition P0 (0%), P1 (0.3%), P2 (0.5%), P3 (1%), and P4 (1.5%). The parameters included reduction sugar, antioxidant activity, water activity, texture, and color (L* a* b*).

Results: The use of propolis on cow’s milk jelly candy has a very significant effect (P<0.01) in reduction sugar, antioxidant activity, water activity, texture, and color (L* a* b*). Increasing the concentration of propolis addition can increase sugar reduction (19.48-20.50%), antioxidant activity (66.72 - 95.67mg/ml), texture (23 - 34 N), color parameters a* ((-1.24) – (-0.65)) and color parameters b* (11.47 - 12.57); while water activity (0.82 - 0.91%), color parameters L* (55.34 - 59.08) decreased in cow’s milk jelly candy.

Conclusion: Treatment P4 is the best treatment, namely having the highest antioxidants 66.72 mg.ml the lowest water activity 0.82 with a higer texture value 34 N. The color of the P4 jelly candy is the lowest Lightness L 55.34 in reddish a* -0.65 and yellowish b* 12.57 higher. Comparable to high reduction sugar 24.50% related to the maillard reaction which can affect the browning of the color.

Keywords: Bee, product, quality, P4 jelly candy, completely randomized design


How to Cite

Husna, Faradisa Zul Alfi, Dedes Amertaningtyas, Khothibul Umam Al Awwaly, Herly Evanuarini, Firman Jaya, Aris Sri Widati, Agus Susilo, Premy Puspitawati Rahayu, and Aulia Puspita Anugra Yekti. 2025. “Application Natural Preservative Use Propolis: Effect on Reduction Sugar, Antioxidant and Physical of Milk Jelly Candy”. Asian Journal of Food Research and Nutrition 4 (4):1561-68. https://doi.org/10.9734/ajfrn/2025/v4i4351.

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