Isolation Identification and Antibiogram of Pseudomonas aeruginosa, Klebsiella pneumonia and Escherichia coli Isolates from Ready to Eat Food Samples in Uli Campus
Published: 30-05-2022
Page: 35-41
Issue: 2022 - Volume 1 [Issue 1]
Ezemba, C. C. *
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Mmaduekwe, C. J.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Udeze, C. P.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Ogumuo, F. C.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Ozoekwe, N.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Duru, B. N.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Ndulue, F. K.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Okoye, P. C.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Udoye, I. W.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Ezemba, A. S.
Department of Microbiology and Brewing, Nnamdi Azikiwe Unversity Awka, Anambra State, Nigeria.
Ogbuagu, S. I.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Ezeigwe C.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Anakwenze, V. N.
Department of Microbiology and Brewing, Nnamdi Azikiwe Unversity Awka, Anambra State, Nigeria.
Ezeokoli, C. M.
Department of Microbiology and Brewing, Nnamdi Azikiwe Unversity Awka, Anambra State, Nigeria.
Osuala O. J.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
Etikudike V. O.
Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Food-borne disease outbreak have imposed substantial burden on health care systems and have markedly reduced the economic productivity of the country. Ready to eat food are well known source for bacteriological contamination. They contain pathogens responsible for health hazard like food poisoning and diarrheal disease. This study aims to evaluate the presence of Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli in of fried rice, jollof rice, turkey and chicken as well as antibiogram profile of the isolates incriminated. A comparative study of the food samples was carried out using standard procedures for isolation and identification of Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli. The antibiotics sensitivity disc was used to determine the antibiogram profile of the isolates. The prevalence of E. coli, K. pneumonia and P. aeruginosa and in food samples was found to be 68.45%, 20.24% and 11.31% respectively. E. coli and K. pneumonia was susceptible to Tarivid 10µg, Reflacine 10µg, Ciprofloxacin 10µg and Streptomycin 30 µg while P. aeruginosa was resistant to the antibiotics used. These data revealed the presence of E. coli, K. pneumonia and P. aeruginosa from different ready to eat foods as well different reactions to certain antibiotic tested.
Keywords: Food safety, antibiogram, food quality, hygiene standards, food microbiology