HPLC Characterization and Sensorial Analysis of Kombucha Based Synbiotic Beverage Infused with Chicory Root Powder (Cichorium intybus)

Anshu *

Department of Food and Nutrition, School of Home Science, Babasaheb Bhimrao Ambedkar University, Lucknow – 226025, India.

Neetu Singh

Department of Food and Nutrition, School of Home Science, Babasaheb Bhimrao Ambedkar University, Lucknow – 226025, India.

Prashant Sagar

Department of Food and Nutrition, School of Home Science, Babasaheb Bhimrao Ambedkar University, Lucknow – 226025, India.

*Author to whom correspondence should be addressed.


Abstract

Probiotic and prebiotic drinks are promoters of the growth and maintenance of healthy intestinal microbiota composition. In spite of that, a product that gathers the medicinal attributes of symbiotic multiple yeast- bacteria colonies, like Kombucha, has not yet been explored and appears to be potentially profitable for human. "Kombucha" is a beverage created by brewing black or green tea and sugar using a SCOBY (Symbiotic Culture of Bacteria and incentive), generally for 7 – 21 days. Quantification of the many constituents that make up our food, whether they are desirable (vitamins, antioxidants, nutrients) or undesirable (pesticides, toxins), is one of the most practical applications of chemistry. A low- sugar kombucha beverage using chicory root powder as fermentation substrate with sensorial acceptability compatible with the traditional Kombucha was developed in the present study and analyzed using high-performance liquid chromatography (HPLC). Kombucha produces acetic acid in addition to a variety of other organic acids and vitamins. The aim of this study was to analyze the acid content of kombucha over a 21-day fermentation period in order to characterize the fermentation kinetics. The organoleptic characteristics such as appearance, odor, flavour, and over all acceptability of the sample were analysed using 9-point hedonic scale. Advancements in the aroma and taste are necessary for acceptable Chicory infused Kombucha beverage.

Keywords: Kombucha, sensory analysis, HPLC, chicory root, SCOBY, chromatography


How to Cite

Anshu, Neetu Singh, and Prashant Sagar. 2023. “HPLC Characterization and Sensorial Analysis of Kombucha Based Synbiotic Beverage Infused With Chicory Root Powder (Cichorium Intybus)”. Asian Journal of Food Research and Nutrition 2 (4):351-58. https://journalajfrn.com/index.php/AJFRN/article/view/58.

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References

Batista P, Penas MR, Pintado M, Oliveira-Silva P. Kombucha: Perceptions and future prospects. Foods. 2022;11(13):1977.

Bishop P, Pitts ER, Budner D, Thompson-Witrick KA. Chemical composition of kombucha. Beverages. 2022;8(3):45.

Jakubczyk K, Kałduńska J, Kochman J, Janda K. Chemical profile and antioxidant activity of the kombucha beverage derived from white, green, black and red tea. Antioxidants. 2020;9(5):447.

Dutta H, Paul SK. Kombucha drink: Production, quality, and safety aspects. In Production and management of beverages. Woodhead Publishing. 2019; 259-288.

Leal JM, Suárez LV, Jayabalan R, Oros JH, Escalante-Aburto A. A review on health benefits of Kombucha nutritional compounds and metabolites. CyTA-Journal of Food, 2018;16(1):390-3.

Khosravi S, Safari M, Emam-Djomeh Z, Golmakani MT. Development of fermented date syrup using Kombucha starter culture. Journal of Food Processing and Preservation. 2019;43(2):e13872.

Available:https://doi.org/10.1111/jfpp.13872

Jayabalan R, Malbaša RV, Lončar ES, Vitas JS, Sathishkumar M. A review on Kombucha tea—microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Comprehensive Reviews in Food Science and Food Safety. 2014;13(4):538-550.

Available:https://doi.org/10.1111/1541-4337.12073

Kapp JM, Sumner W. Kombucha: A systematic review of the empirical evidence of human health benefit. Annals of epidemiology. 2019;30:66-70.

Jayasena V, Cameron I. °Bx/acid ratio as a predictor of consumer acceptability of Crimson Seedless table grapes. Journal of Food Quality. 2008;31(6):736-750.

Available:https://doi.org/10.1111/j.1745- 4557.2008.00231.x

Muzaifa M, Rohaya S, Nilda C, Harahap KR. Kombucha fermentation from cascara with addition of red dragon fruit (Hylocereuspolyrhizus): Analysis of alcohol content and total soluble solid. In International Conference on Tropical Agrifood, Feed and Fuel (ICTAFF 2021) Atlantis Press. 2022 January;125-129.

Vick O, Branan B. Analysis of the antioxidants in homemade vs. commercial kombucha. Journal of Undergraduate Chemistry Research. 2022;21(3):74.

Kumar D, Rai DC, Alam T, Rai D, Bhardwaj A. To study the effect of chicory root extract on physico-chemical properties of synbiotic yoghurt-ice cream. Milk Science International-Milchwissenschaft. 2019;72(4):25-29.

Durães GCS, Pires BAM, Lins TCL. Kombucha based synbiotic beverage using Yacon (Smallanthussonchifolius) as a fermentation substrate: Development and sensorial analysis. Food Research. 2021; 5(4):66-71.

Villarreal‐Soto SA, Beaufort S, Bouajila J, Souchard JP, Taillandier P. Understanding Kombucha tea fermentation: A review. Journal of Food Science. 2018;83(3):580-588.

Available:https://doi.org/10.1111/1750-3841.14068

Miranda B, Lawton NM, Tachibana SR, Swartz NA. Hall WP. Titration and HPLC characterization of kombucha fermentation: A laboratory experiment in food analysis. Journal of Chemical Education. 2016;93(10):1770-1775.