HPLC Characterization and Sensorial Analysis of Kombucha Based Synbiotic Beverage Infused with Chicory Root Powder (Cichorium intybus)
Published: 18-07-2023
Page: 351-358
Issue: 2023 - Volume 2 [Issue 4]
Anshu *
Department of Food and Nutrition, School of Home Science, Babasaheb Bhimrao Ambedkar University, Lucknow – 226025, India.
Neetu Singh
Department of Food and Nutrition, School of Home Science, Babasaheb Bhimrao Ambedkar University, Lucknow – 226025, India.
Prashant Sagar
Department of Food and Nutrition, School of Home Science, Babasaheb Bhimrao Ambedkar University, Lucknow – 226025, India.
*Author to whom correspondence should be addressed.
Abstract
Probiotic and prebiotic drinks are promoters of the growth and maintenance of healthy intestinal microbiota composition. In spite of that, a product that gathers the medicinal attributes of symbiotic multiple yeast- bacteria colonies, like Kombucha, has not yet been explored and appears to be potentially profitable for human. "Kombucha" is a beverage created by brewing black or green tea and sugar using a SCOBY (Symbiotic Culture of Bacteria and incentive), generally for 7 – 21 days. Quantification of the many constituents that make up our food, whether they are desirable (vitamins, antioxidants, nutrients) or undesirable (pesticides, toxins), is one of the most practical applications of chemistry. A low- sugar kombucha beverage using chicory root powder as fermentation substrate with sensorial acceptability compatible with the traditional Kombucha was developed in the present study and analyzed using high-performance liquid chromatography (HPLC). Kombucha produces acetic acid in addition to a variety of other organic acids and vitamins. The aim of this study was to analyze the acid content of kombucha over a 21-day fermentation period in order to characterize the fermentation kinetics. The organoleptic characteristics such as appearance, odor, flavour, and over all acceptability of the sample were analysed using 9-point hedonic scale. Advancements in the aroma and taste are necessary for acceptable Chicory infused Kombucha beverage.
Keywords: Kombucha, sensory analysis, HPLC, chicory root, SCOBY, chromatography
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References
Batista P, Penas MR, Pintado M, Oliveira-Silva P. Kombucha: Perceptions and future prospects. Foods. 2022;11(13):1977.
Bishop P, Pitts ER, Budner D, Thompson-Witrick KA. Chemical composition of kombucha. Beverages. 2022;8(3):45.
Jakubczyk K, Kałduńska J, Kochman J, Janda K. Chemical profile and antioxidant activity of the kombucha beverage derived from white, green, black and red tea. Antioxidants. 2020;9(5):447.
Dutta H, Paul SK. Kombucha drink: Production, quality, and safety aspects. In Production and management of beverages. Woodhead Publishing. 2019; 259-288.
Leal JM, Suárez LV, Jayabalan R, Oros JH, Escalante-Aburto A. A review on health benefits of Kombucha nutritional compounds and metabolites. CyTA-Journal of Food, 2018;16(1):390-3.
Khosravi S, Safari M, Emam-Djomeh Z, Golmakani MT. Development of fermented date syrup using Kombucha starter culture. Journal of Food Processing and Preservation. 2019;43(2):e13872.
Available:https://doi.org/10.1111/jfpp.13872
Jayabalan R, Malbaša RV, Lončar ES, Vitas JS, Sathishkumar M. A review on Kombucha tea—microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Comprehensive Reviews in Food Science and Food Safety. 2014;13(4):538-550.
Available:https://doi.org/10.1111/1541-4337.12073
Kapp JM, Sumner W. Kombucha: A systematic review of the empirical evidence of human health benefit. Annals of epidemiology. 2019;30:66-70.
Jayasena V, Cameron I. °Bx/acid ratio as a predictor of consumer acceptability of Crimson Seedless table grapes. Journal of Food Quality. 2008;31(6):736-750.
Available:https://doi.org/10.1111/j.1745- 4557.2008.00231.x
Muzaifa M, Rohaya S, Nilda C, Harahap KR. Kombucha fermentation from cascara with addition of red dragon fruit (Hylocereuspolyrhizus): Analysis of alcohol content and total soluble solid. In International Conference on Tropical Agrifood, Feed and Fuel (ICTAFF 2021) Atlantis Press. 2022 January;125-129.
Vick O, Branan B. Analysis of the antioxidants in homemade vs. commercial kombucha. Journal of Undergraduate Chemistry Research. 2022;21(3):74.
Kumar D, Rai DC, Alam T, Rai D, Bhardwaj A. To study the effect of chicory root extract on physico-chemical properties of synbiotic yoghurt-ice cream. Milk Science International-Milchwissenschaft. 2019;72(4):25-29.
Durães GCS, Pires BAM, Lins TCL. Kombucha based synbiotic beverage using Yacon (Smallanthussonchifolius) as a fermentation substrate: Development and sensorial analysis. Food Research. 2021; 5(4):66-71.
Villarreal‐Soto SA, Beaufort S, Bouajila J, Souchard JP, Taillandier P. Understanding Kombucha tea fermentation: A review. Journal of Food Science. 2018;83(3):580-588.
Available:https://doi.org/10.1111/1750-3841.14068
Miranda B, Lawton NM, Tachibana SR, Swartz NA. Hall WP. Titration and HPLC characterization of kombucha fermentation: A laboratory experiment in food analysis. Journal of Chemical Education. 2016;93(10):1770-1775.