Quality Evaluation of Biscuits Made from Amaranthus Seed (Amaranthus spp) and Tiger Nut (Cyperus esculentus lativum) Flour Blend.

Ayo J. A. *

Department of Food Science and Technology, Federal University Wukari, Nigeria.

Awogbenja M. D.

Department of Nutrition and Dietetics, Nasarawa State University, Lafia Campus, Lafia, Nigeria.

Gbuusu B.

Department of Food Science and Technology, University of Mkar, Mkar, Nigeria.

Akaahan S. P.

Department of Food Science and Technology, University of Mkar, Mkar, Nigeria.

Gani I. A.

Department of Food Science and Technology, Federal University Wukari, Nigeria.

*Author to whom correspondence should be addressed.


This study investigated the production of flour and biscuit from blends of amaranth grain and tiger nut flours. The blend biscuits produced were analyzed for chemical composition properties. The tiger nut was substituted into amaranth grain flour at 5, 10, 15, 20 and 25% to produce amaranth grain-tiger nut flour blend which was used with other ingredients (fat, salt, baking powder and sugar) baked at 160oC to produce amaranth grain-tiger nut biscuits. The chemical composition, physical and sensory properties of the biscuit were determined [1]. The moisture, protein and carbohydrate content of the blend biscuit decreased from 9.38 to 8.58, 18.90 to 16.68 and 59.97 to 58.17 %, respectively, with the increase in added tiger nut (0 to25%).  The fat, fibre and ash content of the blend biscuit increased from 6.87 to 10.41, 2.22 to 3.23 and 2.26 to 2.99% respectively with increase in added tiger nut flour. The flavonoids, lycopene, β-carotenoid and total carotenod content result increased from 2218.60 to 2277.60, 0.22 to 1.47, 0.93 to 2.23 and 12.79 to 27.19 mg/100g respectively, with increase in added tiger nut flour. The blend biscuit (25% tiger nut) had the highest values of fat, crude fibre and ash (10.41, 3.23 and 2.93%). The tiger nut incorporation had significant effects and could contribute to the improvement of the flour blend biscuits properties. The addition of tiger nut flour to amaranth seed flour in the production of biscuits has relatively improved the nutrient contents, particularly the fibre and phytochemicals (flavonoids, lycopene and β-carotenoid). Also the crispiness of the blend biscuit was greatly improved. The biscuit was accepted up to 25% of the tiger nut flour.

Keywords: Evaluation, biscuits, cream coloured amaranth, tigernut

How to Cite

Ayo J. A., Awogbenja M. D., Gbuusu B., Akaahan S. P., & Gani I. A. (2023). Quality Evaluation of Biscuits Made from Amaranthus Seed (Amaranthus spp) and Tiger Nut (Cyperus esculentus lativum) Flour Blend. Asian Journal of Food Research and Nutrition, 2(4), 681–694. Retrieved from https://journalajfrn.com/index.php/AJFRN/article/view/89


AOAC. (Association of Official Analytical Chemists). Association of official Analytical Chemists, Official Methods o Analysis, AOAC International 19th edn. Washington Dc. 2012;121-130

IDRC - International Development Research Centre. Archived from the original; 2016.

ProdSTAT. FAOSTAT. Archived from the original; 2012. [Accessed on 9 July2020]

Walker CEA, Hazelton JL. Dough rheological tests. Cereal Foods World. 1996;41,23-28.

O’Briena CM, Chapmanb D, Neville DP, Keogh MK, Arendt EK. Effect of varying the micro-encapsulation process on The Nigerian Journal of Nutritional Sciences. March 2011 61 functionality of hydrogenated vegetable fat in short dough biscuits. Fd. Res. Int.; 2003;32(1)36:215–21.

De Castro, O., Gargiulo, R., Del Guacchio, E., Caputo, P. and De Luca, P. A molecular survey concerning theorigin ofCyperus esculentus(Cyperaceae, Poales): Two sides of the same coin (weed vs. crop).Ann. Bot.2015,115, 733–745.

Sánchez-Zapata E, Fernández-Lopez J, Pérez-Alvarez JA. Tigernut (Cyperusesculentus) commercialization: Health aspects, composition, properties and food applications. Comprehensive Reviews in Food Science and Food Safety. 2012;11:366-377.

Bamishaiye EI, Bamishaiye OM. Tigernut. As a plant, its derivatives and benefits. African Journal of Food, Agriculture, Nutrition Development. 2011;11(5):5157-5170.

Van Wyk BE. The potential of South African plants in the development of new food and beverage products. S. Afr. J. Bot. 2011;77:857–868.

Udeozor LO. Tigernut-soy milk drink: Preparation, proximate composition and sensory qualities. International Journal of Food Nutrition and Science. 2012;1(4):18-26.

Akubor PI. Protein, physical and sensory properties of Nigerian snacks food (cake, chin chin and puff puff) prepared from cowpea wheat flour blends. Int. J. Fd. Sci. Tech. 2004;39(4):419-424.

Bosch L, Alegría A, Farré R. RP-HPLC Determination of tiger nut and orgeat amino acid contents: SAGE Publications. Fd Sci.Tech.Int. 2005;11:33-40.

Oladele AK, Aina JO. Chemical composition and functional properties of flour produced from two varieties of tigernut (Cyperus esculentus). African Journal of Biotechnology. 2007;6(21): 2473-2476.

Martinez V. Scientific analysis of effects of tiger nut on heart diseases and related aspects In:Tiger Nut and Health; 2003.

Jaiyeoba KF, Opayemi TA. Development and nutrient assessment of maize based food fortified with tiger nut tuber (Cyperus esculentus). The International Journal of Science & Technoledge. 2016;4(10):189-194.

Berkelaar D, Alemu J. Grain amaranth. ECHO Development Notes 91, ECHO, Florida. 2008;1-5.

Ray T, Roy SC. Genetic diversity of Amaranthus spices from Indo- Gagetic plains reveled by RAPD analysis leading to development of ecotype-specific SCAR marker, J. Hered. 2009;100(3):338-47. DOI: 10.1093/jhered/esn 102.Eup 2008 Dec

Venskutonis PR, Kraujalis P. Nutritional components of Amaranth seeds and vegetables: A review on composition, properties and uses. Comprehensive Reviews in Food Science and Food Safety. 2013;12(4):381–412. DOI: 10.1111/1541-4337.12021

Ayo JA. The effect of amaranth grain flour on the quality of bread. Int. J. Food Prop. 2001;4:341-51.

Silva-Sanches C, Barba dela Rosa AP, Leon-Galvan MF, De Lumen BO, de Leon-Rodriguez A, De Mejìa E. Bioactive peptides in Amaranth (Amaranthushypochondriacus) seed. Journal of Agricultural and Food Chemistry. 2004;56:1233-1240.

Stallknecht GF, Schulz-Schaeffer JR. Amaranth rediscovered.. J. Janick and J.E Simon (eds.). New crops. Wiley, New York. 1993;211-218.

Gamel TH, Linssen JP, Messallem AS, Damir AA, Shekib LA. Effect of seed treatments on thechemical composition of two amaranth species: oil, sugars, fibres, minerals, and vitamins. Journal of the Science of Food and Agriculture. 2014;86:82-89.

Becker R, Wheeler EL, Lorenz K, Stafford AE, Grosjean OK, Betschart AA. A compositional study of amaranth grain. Journal of Food Science. 1981;46(4):1175-1180.

Shevkani K, Singh N, Rana JC, Kaur A. Physicochemical, pasting and functional properties of amaranth seed flours: Effects of lipid removal. Journal of Food Science. 2014;79(7):1271-1277.

Ayo A, Nkama I, Ibrahim A. Effect of achaflour on feed intake, body Weight, haematological and nutritional quality ofrabit. Journal of Research in Bioscience. 2007;3(3):76-8

Ayo JA, Gidado FE. Physicochemical, phytochemical and sensory evaluation of acha carrot flours blend biscuit current. Journal of Applied Science and Technology. Article no. CJAST.38142 Previously known as British Journal of Applied Science & Technology. 2017;25(5):1-15. ISSN: 2231-0843, NLM ID: 101664541

Obinna-Echem PC, Robinson ES. Proximatecomposition, physicalandsensory properties of biscuits produced from blends of maize (Zeamays)and tiger nut (Cyperus esculentus) flour. Sky Journal of FoodScience. 2019;7(2):030-036.

Hossain MA, Muhammad MD, Charles G, and Muhammad I. In vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah. Asian Pacific Journal ofTropical Medicine. 2011;4(9):717-721.

Branisa J, Jenisowa Z, Porubska M, Jomova K, Valko M. Spectrophotomeic determination of chlorophylls and carotenoids, an effect of sonication and sample processing. Journal of Microbiology, Biotechnology and Food Science. 2014;3(2):61-64.

Harborne JB. Phytochemical Methods. Chapman and Hall Ltd., Londonpp. 1973;49-188.

Latta M, Eskin M. A simple and rapid colorimetric determination of phytate determination. J. Agric. Food Chem. 1980;28:1313–1315.

Vaintraub IA, Lapteva NA. Colorimetric determination of phytate in unpurifi ed extracts of seeds and the products of their processing. Anal. Biochem. 1988;175:227–230.

Ayo JA, Ojo MA, Popoola CA, Ayo VA, Okpasu A. Production and quality evaluation of acha-tigernut composite flour and biscuits. Asian Food Science Journal. 2018;1(3):1-12.

Ade-Omowaye BI, Akinwande BA, Bolarinwa IF, Adebiyi AO. Evaluation of tiger nut (Cyperus esculentus) - wheat composite flour and bread. Afr.J. Food Sci. 2008;2:087-091.

Bogert JL, Briggs GM, Galloway DH. Nutritional and Physical fitness. Int. Food Sc. Nutri. 1994;45:225-230.

Schnetzler KA, Breene WM. Food uses and amaranth product research: a comprehensive review. In:PeredesLópez O (ed), Amaranth biology, chemistry and technology. CRC Press, Boca Raton, FL. 1994;155-84.

Akujobi I. Nutrient composition and sensory evaluation of cookies produced from cocoa yam (Xanthosoma sagittifoliun) and tiger nut (cyperus esculentus) flour blends. International journal of innovative food, nutrition and sustainable agriculture. 2018;6(3):33- 39.

Muyonga JH, Andabati B, Ssepuya G. Effects of heat processing on selected grain amaranth physico-chemical properties. Food Science and Nutrition. 2014;2(1):9-16. DOI: 10.10002/fsn 3-75

Adebowale AA, Sanni SA, Oladopo FO. Chemical, function and sensory properties of instant yam breadfruit flour. Nigerian Food J. 2008;26(1):2-12

Souleymane B, Patrice B, Gouyahali S, Moe Thida K, Brian P, Stephen N. “Physiochemical characteristic and composition of three morphotypes of ceyperus esculentus tubers and tuber oils. Jounal of analytical methods in chemistry. 2015;2015. DOI: 10.155/2015/673547

Tang Y, Tsao R. Phytochemicals in quinoa and amaranth grains and their antioxidant, anti-inflammatory, and potential health beneficial effects: A review. Mol Nutr Food Res. 2016;61(7) DOI: 10.1002/mnfr.00767 Epub 2017 Apr 18. PMID: 28239982

Chukwuma ER, Obioma N, Christopher OI. The phytochemical composition and some biochemical effects of Nigeria Tiger nut Tuber. Pakistan Journal of Nutrition. 2010;9(7):709-715.

Souci SW, Fachmann W, Kraut H. Food composition and nutrition tables, 5th revised and completed edition. Medpharm, Stuttgart Medpharm GmbH, Scientific Publishers, Stuttgart and CRC Press, Boca Raton, FL. 1994; .

Berghofer E, Schoenlenchner R. Grain Amaranth. In: Belton P, Taylor J (eds.), Pseudocereals and less common cereals, grain properties and Utilization potential. Springer Verlag. 2002;219-60.

Chinma CE, Adewuyi AO, Abu JO. Effect of germination on the chemical, functional and pasting properties of flour from brown and yellow varieties of tigernut. Fd. Res. Int. 2011;42(8):1004-1009.